RESUMO
A one-step modification ofimmunoenzyme assay (dot variant) is proposed for the detection of ABH-Lewis antigens in human discharges (saliva, sperm, vaginal discharge). Sensitivity and specificity of the new method is higher than those of other methods currently used in forensic medicine for the detection of antigens in human body discharges; its other advantages over routine techniques include procedural simplicity, high performance, and the possibility of conducting series measurements with minimal labour inputs. Moreover, it provides information not only about group characteristic but also about category of discharge. The analysis is carried out in supernatant fractions which permits to reserve cellular material for molecular-genetic studies.
Assuntos
Sistema ABO de Grupos Sanguíneos/análise , Secreções Corporais/imunologia , Medicina Legal/métodos , Técnicas Imunoenzimáticas/métodos , Antígenos do Grupo Sanguíneo de Lewis/análise , Sistema ABO de Grupos Sanguíneos/imunologia , Feminino , Humanos , Antígenos do Grupo Sanguíneo de Lewis/imunologia , Masculino , Saliva/química , Saliva/imunologiaRESUMO
A method is proposed for ABO grouping of human hair microfragments 1.5-2.0 cm in length. The method is a modification of the absorption-elution reaction (AER) for monoclonal antibodies (MCA). MCA manufactured by Gematolog Ltd. (Russia) as well as Bioclone anti-A and anti-B reagents (Ortho-Clinical Diagnostics, USA) proved suitable for hair typing. A series of procedures for sample preparation and treatment in the reaction, such as the heating of a hair stem on a boiling water bath and the addition of a non-ionic detergent (Tween 20) to the working solution, were used to prevent non-specific results of the analysis. It is emphasized that each analysis must be made in duplicate.
Assuntos
Sistema ABO de Grupos Sanguíneos , Anticorpos Monoclonais , Tipagem e Reações Cruzadas Sanguíneas/métodos , Medicina Legal/métodos , Cabelo , Sistema ABO de Grupos Sanguíneos/imunologia , Cabelo/imunologia , Humanos , Sensibilidade e EspecificidadeRESUMO
Monoclonal antimorphine antibodies both free and conjugated with horse- radish peroxidase have been raised and used to develop an assay kit for the detection of narcotic opiate-based drugs by an immuno-enzyme assay (IEA). The kit contains all ingredients necessary for the enzymatic reaction. A total of 215 urine and blood samples were analysed using the new kit. The results were compared with the data obtained by thin layer chromatography and high-performance liquid chromatography. False negative results were absent while false positive (inconclusive) results were recorded in three cases, probably due to the fact that sensitivity of IEA is higher than that of control methods. It is concluded that the kit may be used in laboratory screening studies for detecting opiates in biological fluids.
Assuntos
Analgésicos Opioides/sangue , Analgésicos Opioides/urina , Transtornos Relacionados ao Uso de Opioides/sangue , Transtornos Relacionados ao Uso de Opioides/urina , Detecção do Abuso de Substâncias/métodos , Animais , Anticorpos Monoclonais , Calibragem , Feminino , Peroxidase do Rábano Silvestre/química , Humanos , Técnicas Imunoenzimáticas/instrumentação , Técnicas Imunoenzimáticas/métodos , Camundongos , Camundongos Endogâmicos BALB C , Morfina/imunologia , Kit de Reagentes para Diagnóstico , Sensibilidade e Especificidade , Detecção do Abuso de Substâncias/instrumentaçãoRESUMO
The authors studied the concentrations of tryptophan, serotonin and 5-hydroxyindole acetic acid in both blood plasma and cerebrospinal fluid of patients with suppurative meningitides and meningoencephalitides of varying severity. The data obtained point to the intensification of serotonin metabolism in the central nervous system at the height of the disease development which may lead to alterations in the functional activity of serotoninergic neurones.
